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--- sample_collection_and_dna_extraction [2012/02/22 16:14]
csbq_qcbs
+++ sample_collection_and_dna_extraction [2013/11/20 15:24] (current)
anniearchambault
@@ Line 16: / Line 16: @@
 ===Guidelines for preserving DNA integrity in biological samples===
-The guidelines listed below, organized according to the type of organism, follow recommendations from Nagy 2010((Nagy, Z.T., 2010. A hands-on overview of tissue preservation methods for molecular genetic analyses. Organisms Diversity & Evolution 10, 91–105[[http://www.springerlink.com/content/c81h415005u27534/fulltext.html|10.1007/s13127-010-0012-4]])) and from QCBS members and students. In addition to the diverse methods developed, universal commercial solutions, such as [[http://www.qiagen.com/products/rnastabilizationpurification/rnalaterrnaprotectsystems/rnalaterrnastabilization.aspx#Tabs=t0|RNAlater]], are now available for any type of samples or organisms.
+The guidelines listed below, organized according to the type of organism, follow recommendations from Nagy 2010((Nagy, Z.T., 2010. A hands-on overview of tissue preservation methods for molecular genetic analyses. Organisms Diversity & Evolution 10, 91–105[[http://www.springerlink.com/content/c81h415005u27534/fulltext.html|10.1007/s13127-010-0012-4]])) and from QCBS members and students. In addition to the diverse methods developed, universal commercial solutions, such as [[http://www.qiagen.com/products/rnastabilizationpurification/rnalaterrnaprotectsystems/rnalaterrnastabilization.aspx#Tabs=t0|RNAlater]], are now available for any type of samples or organisms. The RNAlater solution is reported to be made of 25 mM Sodium citrate trisodium salt dihydrate, 10 mM EDTA, 70 g/100 ml Ammonium Sulfate; adjusted to a pH of 5.2 with 1M H<sub>2</sub>SO<sub>4</sub>.
 ==Small to large animals==