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methodes_en_biologie_moleculaire [2012/05/17 15:43] anniearchambault |
methodes_en_biologie_moleculaire [2014/11/13 15:54] (current) sebastien.renaut |
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| - | Different molecular biology topics and methods useful in biodiversity science: | + | Different molecular biology topics and methods useful in biodiversity science. |
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| + | ===In the lab=== | ||
| *[[Setting up a molecular biology laboratory|Setting up a laboratory for molecular biology]] | *[[Setting up a molecular biology laboratory|Setting up a laboratory for molecular biology]] | ||
| *[[Sample collection and DNA extraction|Good practices for sample collection; and DNA extraction methods]] | *[[Sample collection and DNA extraction|Good practices for sample collection; and DNA extraction methods]] | ||
| - | *[[Real time PCR|Quantitative real time PCR]], usually form cDNA | + | *[[Real time PCR|Quantitative real time PCR]], usually from cDNA |
| + | *[[High throughput sequencing for genetic diversity|High throughput sequencing for genetic diversity]] | ||
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| + | ===At the computer=== | ||
| *Basic notions in [[BLAST introduction|sequence similarity search]] using BLAST programs | *Basic notions in [[BLAST introduction|sequence similarity search]] using BLAST programs | ||
| - | *[[Sequence similarity search|Sequence similarity search]], and tips on parsing large BLAST output files | + | *[[Standalone BLAST|Standalone BLAST on a local database]], and tips on parsing large BLAST output files |
| - | *Sequence similarity search with [[Batch Blast to the NCBI online database|batch Blast to NCBI]], and parsing the output file | + | *[[Commandline remote BLAST|Commandline multiple BLAST searches on the public NCBI database]] |
| *[[Bioinformatic tools to detect microsatellites loci from genomic data|Bioinformatic tools to detect microsatellites loci from genomic data]] | *[[Bioinformatic tools to detect microsatellites loci from genomic data|Bioinformatic tools to detect microsatellites loci from genomic data]] | ||
| - | *[[High throughput sequencing for genetic diversity|High throughput sequencing for genetic diversity]] | + | *[[Guide to amplicon sequencing experiment|A guide to amplicon sequencing experiments]] for microbial communities analyses |
| + | *[[NGS sequence alignment and variant calling|High throughput sequencing (bioinformatic tools)]] | ||
