Differences
This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision | ||
methodes_en_biologie_moleculaire [2012/05/17 15:48] anniearchambault |
methodes_en_biologie_moleculaire [2014/11/13 15:54] sebastien.renaut |
||
---|---|---|---|
Line 1: | Line 1: | ||
- | Different molecular biology topics and methods useful in biodiversity science: | + | Different molecular biology topics and methods useful in biodiversity science. |
+ | |||
+ | ===In the lab=== | ||
*[[Setting up a molecular biology laboratory|Setting up a laboratory for molecular biology]] | *[[Setting up a molecular biology laboratory|Setting up a laboratory for molecular biology]] | ||
*[[Sample collection and DNA extraction|Good practices for sample collection; and DNA extraction methods]] | *[[Sample collection and DNA extraction|Good practices for sample collection; and DNA extraction methods]] | ||
- | *[[Real time PCR|Quantitative real time PCR]], usually form cDNA | + | *[[Real time PCR|Quantitative real time PCR]], usually from cDNA |
+ | *[[High throughput sequencing for genetic diversity|High throughput sequencing for genetic diversity]] | ||
+ | |||
+ | |||
+ | ===At the computer=== | ||
*Basic notions in [[BLAST introduction|sequence similarity search]] using BLAST programs | *Basic notions in [[BLAST introduction|sequence similarity search]] using BLAST programs | ||
- | *[[Standalone BLAST|Standalone BLAST on a local database]], and tips on parsing large BLAST output files | + | *[[Standalone BLAST|Standalone BLAST on a local database]], and tips on parsing large BLAST output files |
- | *Sequence similarity search with [[Batch Blast to the NCBI online database|batch Blast to NCBI]], and parsing the output file | + | *[[Commandline remote BLAST|Commandline multiple BLAST searches on the public NCBI database]] |
*[[Bioinformatic tools to detect microsatellites loci from genomic data|Bioinformatic tools to detect microsatellites loci from genomic data]] | *[[Bioinformatic tools to detect microsatellites loci from genomic data|Bioinformatic tools to detect microsatellites loci from genomic data]] | ||
- | *[[High throughput sequencing for genetic diversity|High throughput sequencing for genetic diversity]] | + | *[[Guide to amplicon sequencing experiment|A guide to amplicon sequencing experiments]] for microbial communities analyses |
+ | *[[NGS sequence alignment and variant calling|High throughput sequencing (bioinformatic tools)]] |