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methodes_en_biologie_moleculaire [2012/05/17 15:38] anniearchambault |
methodes_en_biologie_moleculaire [2014/11/13 15:54] sebastien.renaut |
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- | Different molecular biology topics and methods useful in biodiversity science: | + | Different molecular biology topics and methods useful in biodiversity science. |
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+ | ===In the lab=== | ||
*[[Setting up a molecular biology laboratory|Setting up a laboratory for molecular biology]] | *[[Setting up a molecular biology laboratory|Setting up a laboratory for molecular biology]] | ||
*[[Sample collection and DNA extraction|Good practices for sample collection; and DNA extraction methods]] | *[[Sample collection and DNA extraction|Good practices for sample collection; and DNA extraction methods]] | ||
- | *[[Real time PCR|Quantitative real time PCR]], usually form cDNA | + | *[[Real time PCR|Quantitative real time PCR]], usually from cDNA |
- | *[[Sequence similarity search|Sequence similarity search]], and tips on parsing large BLAST output files | + | |
- | *[[Batch Blast to the NCBI online database|Batch Blast to the NCBI online database]], and parsing the output file | + | |
- | *[[Bioinformatic tools to detect microsatellites loci from genomic data|Bioinformatic tools to detect microsatellites loci from genomic data]] | + | |
*[[High throughput sequencing for genetic diversity|High throughput sequencing for genetic diversity]] | *[[High throughput sequencing for genetic diversity|High throughput sequencing for genetic diversity]] | ||
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+ | ===At the computer=== | ||
+ | *Basic notions in [[BLAST introduction|sequence similarity search]] using BLAST programs | ||
+ | *[[Standalone BLAST|Standalone BLAST on a local database]], and tips on parsing large BLAST output files | ||
+ | *[[Commandline remote BLAST|Commandline multiple BLAST searches on the public NCBI database]] | ||
+ | *[[Bioinformatic tools to detect microsatellites loci from genomic data|Bioinformatic tools to detect microsatellites loci from genomic data]] | ||
+ | *[[Guide to amplicon sequencing experiment|A guide to amplicon sequencing experiments]] for microbial communities analyses | ||
+ | *[[NGS sequence alignment and variant calling|High throughput sequencing (bioinformatic tools)]] |