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aflp_454seq_for_pop_structure [2012/02/02 14:42] anniearchambault |
aflp_454seq_for_pop_structure [2012/02/02 15:51] (current) anniearchambault |
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| <WRAP box 500px>**Table 5** Reaction mix for selective amplifications, in the context of a modified AFLP method for genome complexity reduction coupled to multiplexing samples for high throughput sequencing. | <WRAP box 500px>**Table 5** Reaction mix for selective amplifications, in the context of a modified AFLP method for genome complexity reduction coupled to multiplexing samples for high throughput sequencing. | ||
| ^ Reagent ^ Initial conc. ^ Qty added ^ Final conc. or Final qty ^ | ^ Reagent ^ Initial conc. ^ Qty added ^ Final conc. or Final qty ^ | ||
| - | ^ HF Buffer | 5X includes (15 mM MgCl2) | 6 µl | 1X | | + | ^ HF Buffer | 5X (includes 15 mM MgCl2) | 6 µl | 1X | |
| ^ MgCl2 | 50 mM | 0.6 µl | 2.5 mM | | ^ MgCl2 | 50 mM | 0.6 µl | 2.5 mM | | ||
| ^ dNTP | 10 mM | 0.6 µl | 200 µM | | ^ dNTP | 10 mM | 0.6 µl | 200 µM | | ||
