Different molecular biology topics and methods useful in biodiversity science.
In the lab
Setting up a laboratory for molecular biology
Good practices for sample collection; and DNA extraction methods
Quantitative real time PCR
, usually from cDNA
High throughput sequencing for genetic diversity
At the computer
Basic notions in
sequence similarity search
using BLAST programs
Standalone BLAST on a local database
, and tips on parsing large BLAST output files
Commandline multiple BLAST searches on the public NCBI database
Bioinformatic tools to detect microsatellites loci from genomic data
A guide to amplicon sequencing experiments
for microbial communities analyses
High throughput sequencing (bioinformatic tools)